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  • 名 称:
    DYRK2/4 抗体
  • 货 号:
    DF10330
  • RRID:
    AB_2844337
  • 来 源:
    Rabbit
  • 应 用:
    WB,ELISA(peptide)
  • 反 应:
    Human
  • 预 测:
    Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
  • 蛋白号:
  • 分子量:
    60kD;
    67kDa,60kDa(Calculated).
  • 浓 度:
    1mg/ml
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产品信息

别名:Expand▼

1810038L18Rik; Dual specificity tyrosine (Y) phosphorylation regulated kinase 2; Dual specificity tyrosine phosphorylation regulated kinase 2; Dual specificity tyrosine-phosphorylation-regulated kinase 2; DYRK2; DYRK2_HUMAN; EC 2.7.12.1; FLJ21217; FLJ21365; Dual specificity tyrosine phosphorylation regulated kinase 4; Dual specificity tyrosine-phosphorylation-regulated kinase 4; Dyrk4; DYRK4_HUMAN; EC 2.7.12.1;

应用:

WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.

反应性:

Human

预测反应:

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)

来源:

Rabbit

克隆性:

Polyclonal

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

特异性:

DYRK2/4 Antibody detects endogenous levels of total DYRK2/4.

免疫原:

A synthesized peptide derived from human DYRK2/4, corresponding to a region within the internal amino acids.

RRID:

AB_2844337
引用格式: Affinity Biosciences Cat# DF10330, RRID:AB_2844337.

形式:

Liquid

浓度:

1mg/ml

保存条件:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

免疫原

Immunogen:

A synthesized peptide derived from human DYRK2/4, corresponding to a region within the internal amino acids.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

DYRK2,DYRK4

Molecular Weight:

Observed Mol.Wt.: 60kD.
Predicted Mol.Wt.: 67kDa,60kDa(Calculated)..

Subcellular Location:

Cytoplasm. Nucleus. Translocates into the nucleus following DNA damage.

Tissue Specificity:

Q92630 DYRK2_HUMAN:
Testis, after the onset of spermatogenesis.

Sequence:
MLTRKPSAAAPAAYPTGRGGDSAVRQLQASPGLGAGATRSGVGTGPPSPIALPPLRASNAAAAAHTIGGSKHTMNDHLHVGSHAHGQIQVQQLFEDNSNKRTVLTTQPNGLTTVGKTGLPVVPERQLDSIHRRQGSSTSLKSMEGMGKVKATPMTPEQAMKQYMQKLTAFEHHEIFSYPEIYFLGLNAKKRQGMTGGPNNGGYDDDQGSYVQVPHDHVAYRYEVLKVIGKGSFGQVVKAYDHKVHQHVALKMVRNEKRFHRQAAEEIRILEHLRKQDKDNTMNVIHMLENFTFRNHICMTFELLSMNLYELIKKNKFQGFSLPLVRKFAHSILQCLDALHKNRIIHCDLKPENILLKQQGRSGIKVIDFGSSCYEHQRVYTYIQSRFYRAPEVILGARYGMPIDMWSLGCILAELLTGYPLLPGEDEGDQLACMIELLGMPSQKLLDASKRAKNFVSSKGYPRYCTVTTLSDGSVVLNGGRSRRGKLRGPPESREWGNALKGCDDPLFLDFLKQCLEWDPAVRMTPGQALRHPWLRRRLPKPPTGEKTSVKRITESTGAITSISKLPPPSSSASKLRTNLAQMTDANGNIQQRTVLPKLVS

MPASELKASEIPFHPSIKTQDPKAEEKSPKKQKVTLTAAEALKLFKNQLSPYEQSEILGYAELWFLGLEAKKLDTAPEKFSKTSFDDEHGFYLKVLHDHIAYRYEVLETIGKGSFGQVAKCLDHKNNELVALKIIRNKKRFHQQALMELKILEALRKKDKDNTYNVVHMKDFFYFRNHFCITFELLGINLYELMKNNNFQGFSLSIVRRFTLSVLKCLQMLSVEKIIHCDLKPENIVLYQKGQASVKVIDFGSSCYEHQKVYTYIQSRFYRSPEVILGHPYDVAIDMWSLGCITAELYTGYPLFPGENEVEQLACIMEVLGLPPAGFIQTASRRQTFFDSKGFPKNITNNRGKKRYPDSKDLTMVLKTYDTSFLDFLRRCLVWEPSLRMTPDQALKHAWIHQSRNLKPQPRPQTLRKSNSFFPSETRKDKVQGCHHSSRKADEITKETTEKTKDSPTKHVQHSGDQQDCLQHGADTVQLPQLVDAPKKSEAAVGAEVSMTSPGQSKNFSLKNTNVLPPIV

研究背景

Function:

Serine/threonine-protein kinase involved in the regulation of the mitotic cell cycle, cell proliferation, apoptosis, organization of the cytoskeleton and neurite outgrowth. Functions in part via its role in ubiquitin-dependent proteasomal protein degradation. Functions downstream of ATM and phosphorylates p53/TP53 at 'Ser-46', and thereby contributes to the induction of apoptosis in response to DNA damage. Phosphorylates NFATC1, and thereby inhibits its accumulation in the nucleus and its transcription factor activity. Phosphorylates EIF2B5 at 'Ser-544', enabling its subsequent phosphorylation and inhibition by GSK3B. Likewise, phosphorylation of NFATC1, CRMP2/DPYSL2 and CRMP4/DPYSL3 promotes their subsequent phosphorylation by GSK3B. May play a general role in the priming of GSK3 substrates. Inactivates GYS1 by phosphorylation at 'Ser-641', and potentially also a second phosphorylation site, thus regulating glycogen synthesis. Mediates EDVP E3 ligase complex formation and is required for the phosphorylation and subsequent degradation of KATNA1. Phosphorylates TERT at 'Ser-457', promoting TERT ubiquitination by the EDVP complex. Phosphorylates SIAH2, and thereby increases its ubiquitin ligase activity. Promotes the proteasomal degradation of MYC and JUN, and thereby regulates progress through the mitotic cell cycle and cell proliferation. Promotes proteasomal degradation of GLI2 and GLI3, and thereby plays a role in smoothened and sonic hedgehog signaling. Plays a role in cytoskeleton organization and neurite outgrowth via its phosphorylation of DCX and DPYSL2. Phosphorylates CRMP2/DPYSL2, CRMP4/DPYSL3, DCX, EIF2B5, EIF4EBP1, GLI2, GLI3, GYS1, JUN, MDM2, MYC, NFATC1, p53/TP53, TAU/MAPT and KATNA1. Can phosphorylate histone H1, histone H3 and histone H2B (in vitro). Can phosphorylate CARHSP1 (in vitro).

Post-translational Modifications:

Autophosphorylates cotranslationally on the second tyrosine residue in the Tyr-X-Tyr motif in the activation loop, but once mature, does not have any protein tyrosine kinase activity. Phosphorylated at Thr-106 and Ser-442 by ATM in response to genotoxic stress.

Under normal conditions, polyubiquitinated in the nucleus by MDM2, leading to its proteasomal degradation. Phosphorylation on Thr-106 and Ser-442 by ATM in response to genotoxic stress disrupts MDM2 binding and prevents MDM2-mediated ubiquitination and subsequent proteasomal degradation. Polyubiquitinated by SIAH2, leading to its proteasomal degradation. Polyubiquitinated by SIAH2 occurs under normal conditions, and is enhanced in response to hypoxia.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Translocates into the nucleus following DNA damage.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location

Tissue Specificity:

Testis, after the onset of spermatogenesis.

Subunit Structure:

Component of an E3 ligase complex containing DYRK2, EDD/UBR5, DDB1 and DCAF1 (EDVP complex). Interacts directly with EDD/UBR5, DDB1 and DCAF1. Interacts with SIAH2 and MDM2. Interacts with MAP3K10 and NFATC1. May also interact with CCNL2.

Similarity:

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.

Function:

Possible non-essential role in spermiogenesis.

Post-translational Modifications:

Autophosphorylation on Tyr-264 in the activation loop is required for kinase activity.

Subcellular Location:

Cytoplasm.

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location

Similarity:

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.

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Catalog Number :

DF10330-BP
(Blocking peptide available as DF10330-BP)

Price/Size :

¥2000/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Zebrafish
100%
Chicken
100%
Rabbit
100%
Pig
100%
Dog
100%
Horse
100%
Bovine
100%
Sheep
100%
Xenopus
0%
High similarity Medium similarity Low similarity No similarity
Q92630/Q9NR20 as Substrate
Site PTM Type Enzyme
T37 Phosphorylation
K71 Ubiquitination
K72 Ubiquitination
T211 Phosphorylation
S213 Phosphorylation
S222 Phosphorylation
Y262 Phosphorylation
T263 Phosphorylation
Y264 Phosphorylation Q9NR20 (DYRK4)
S267 Phosphorylation
Y356 Phosphorylation
K360 Sumoylation
K417 Ubiquitination
S437 Phosphorylation
T445 Phosphorylation
T457 Phosphorylation
Site PTM Type Enzyme
Ubiquitination
S7 Phosphorylation
R18 Methylation
S30 Phosphorylation
T44 Phosphorylation
S48 Phosphorylation
T106 Phosphorylation Q13315 (ATM)
S142 Phosphorylation
C347 S-Nitrosylation
C373 S-Nitrosylation
Y380 Phosphorylation
T381 Phosphorylation Q02779 (MAP3K10)
Y382 Phosphorylation Q92630 (DYRK2)
S385 Phosphorylation
S442 Phosphorylation Q13315 (ATM)
S449 Phosphorylation Q02779 (MAP3K10)
Y464 Phosphorylation
T525 Phosphorylation
Q92630/Q9NR20 as PTM Enzyme
Substrate Site Source
Q9NR20 (DYRK4) Y264 Uniprot
Substrate Site Source
O43255 (SIAH2) S16 Uniprot
O43255 (SIAH2) T26 Uniprot
O43255 (SIAH2) S28 Uniprot
O43255 (SIAH2) S68 Uniprot
O43255 (SIAH2) T119 Uniprot
O43602-2 (DCX) S306 Uniprot
O75449 (KATNA1) S42 Uniprot
O75449 (KATNA1) S109 Uniprot
O75449 (KATNA1) T133 Uniprot
P01106 (MYC) S62 Uniprot
P04637 (TP53) S46 Uniprot
P05412 (JUN) S243 Uniprot
P10070 (GLI2) S388 Uniprot
P10070 (GLI2) S1011 Uniprot
P10636-8 (MAPT) T212 Uniprot
P10636 (MAPT) T529 Uniprot
P13807 (GYS1) S641 Uniprot
P43686 (PSMC4) T25 Uniprot
Q13541 (EIF4EBP1) S65 Uniprot
Q13541 (EIF4EBP1) S101 Uniprot
Q14194 (CRMP1) S522 Uniprot
Q14195 (DPYSL3) T509 Uniprot
Q14195 (DPYSL3) T514 Uniprot
Q14195 (DPYSL3) S518 Uniprot
Q14195 (DPYSL3) S522 Uniprot
Q16555 (DPYSL2) S522 Uniprot
Q92630 (DYRK2) Y382 Uniprot
Q9UHD2 (TBK1) S527 Uniprot
Q9Y2V2 (CARHSP1) S30 Uniprot
Q9Y2V2 (CARHSP1) S32 Uniprot
Q9Y2V2 (CARHSP1) S41 Uniprot
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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