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  • 名 称:
    磷酸化 HNF4 alpha (Ser313) 抗体
  • 货 号:
    AF3297
  • RRID:
    AB_2834716
  • 来 源:
    Rabbit
  • 应 用:
    WB,IHC,IF/ICC,ELISA(peptide)
  • 反 应:
    Human, Mouse, Rat
  • 预 测:
    Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
  • 蛋白号:
  • 分子量:
    52kD;
    53kDa(Calculated).
  • 浓 度:
    1mg/ml
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产品信息

别名:Expand▼

FLJ39654; FRTS4; Hepatic nuclear factor 4 alpha; Hepatocyte nuclear factor 4 alpha; Hepatocyte nuclear factor 4; Hepatocyte nuclear factor 4-alpha; HNF 4 alpha; HNF 4; HNF-4-alpha; HNF4; HNF4A; HNF4A_HUMAN; HNF4a7; HNF4a8; HNF4a9; Hnf4alpha; HNF4alpha10/11/12; MODY 1; MODY; MODY1; NR2A1; NR2A21; Nuclear receptor subfamily 2 group A member 1; OTTHUMP00000031060; OTTHUMP00000031062; TCF 14; TCF; TCF-14; TCF14; Tcf4; Transcription factor 14, hepatic nuclear factor; Transcription factor 14; Transcription factor HNF 4; Transcription factor HNF-4; Transcription factor HNF4;

应用:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.

反应性:

Human, Mouse, Rat

预测反应:

Pig(100%), Zebrafish(82%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(82%), Xenopus(82%)

来源:

Rabbit

克隆性:

Polyclonal

纯化:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

特异性:

Phospho-HNF4 alpha (Ser313) Antibody detects endogenous levels of HNF4 alpha only when phosphorylated at Serine 313.

RRID:

AB_2834716
引用格式: Affinity Biosciences Cat# AF3297, RRID:AB_2834716.

形式:

Liquid

浓度:

1mg/ml

保存条件:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

免疫原

Immunogen:

A synthesized peptide derived from human HNF4 alpha around the phosphorylation site of Ser313.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

HNF4A

Molecular Weight:

Observed Mol.Wt.: 52kD.
Predicted Mol.Wt.: 53kDa(Calculated)..

Subcellular Location:

Nucleus.

Description:

The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes.

Sequence:
MRLSKTLVDMDMADYSAALDPAYTTLEFENVQVLTMGNDTSPSEGTNLNAPNSLGVSALCAICGDRATGKHYGASSCDGCKGFFRRSVRKNHMYSCRFSRQCVVDKDKRNQCRYCRLKKCFRAGMKKEAVQNERDRISTRRSSYEDSSLPSINALLQAEVLSRQITSPVSGINGDIRAKKIASIADVCESMKEQLLVLVEWAKYIPAFCELPLDDQVALLRAHAGEHLLLGATKRSMVFKDVLLLGNDYIVPRHCPELAEMSRVSIRILDELVLPFQELQIDDNEYAYLKAIIFFDPDAKGLSDPGKIKRLRSQVQVSLEDYINDRQYDSRGRFGELLLLLPTLQSITWQMIEQIQFIKLFGMAKIDNLLQEMLLGGSPSDAPHAHHPLHPHLMQEHMGTNVIVANTMPTHLSNGQMCEWPRPRGQAATPETPQPSPPGGSGSEPYKLLPGAVATIVKPLSAIPQPTITKQEVI

研究背景

Function:

Transcriptional regulator which controls the expression of hepatic genes during the transition of endodermal cells to hepatic progenitor cells, facilitating the recruitment of RNA pol II to the promoters of target genes. Activates the transcription of CYP2C38 (By similarity). Represses the CLOCK-ARNTL/BMAL1 transcriptional activity and is essential for circadian rhythm maintenance and period regulation in the liver and colon cells.

Post-translational Modifications:

Phosphorylated on tyrosine residue(s); phosphorylation is important for its DNA-binding activity. Phosphorylation may directly or indirectly play a regulatory role in the subnuclear distribution. Phosphorylation at Ser-313 by AMPK reduces the ability to form homodimers and bind DNA.

Acetylation at Lys-458 lowers transcriptional activation by about two-fold.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location

Subunit Structure:

Homodimerization is required for HNF4-alpha to bind to its recognition site. Interacts with CLOCK, ARNTL, CRY1, CRY2, PER1 and PER2. Interacts with NR0B2/SHP; the resulting heterodimer is transcriptionnally inactive. Interacts with DDX3X; this interaction disrupts the interaction between HNF4 and NR0B2 that forms inactive heterodimers and enhances the formation of active HNF4 homodimers.

Similarity:

The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.

Belongs to the nuclear hormone receptor family. NR2 subfamily.

研究领域

Research Fields:

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.(View pathway)
· Human Diseases > Endocrine and metabolic diseases > Maturity onset diabetes of the young.

WB Images(1)


IHC Images(1)


IF/ICC Images(1)


Peptide-ELISA

ELISA analysis of AF3297 showing specificity to Phospho-HNF4 alpha (Ser313) Blocking Peptide peptide. Peptides concentration: 1ug/ml.
P-peptide: phospho-peptide; N-peptide: non-phospho-peptide.
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Catalog Number :

AF3297-BP
(Blocking peptide available as AF3297-BP)

Price/Size :

¥2000/1mg.
Tips: For modified antibodies, we provide modified peptides(0.5mg) and non-modified peptides(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Pig
100%
Horse
100%
Bovine
100%
Sheep
100%
Dog
100%
Rabbit
100%
Xenopus
82%
Zebrafish
82%
Chicken
82%
High similarity Medium similarity Low similarity No similarity
P41235 as Substrate
Site PTM Type Enzyme
Y23 Phosphorylation P12931 (SRC)
Y72 Phosphorylation
S75 Phosphorylation
S76 Phosphorylation
S95 Phosphorylation
S99 Phosphorylation
K106 Acetylation
K108 Acetylation
K126 Acetylation
K127 Acetylation
S138 Phosphorylation
T139 Phosphorylation
S142 Phosphorylation P17612 (PRKACA)
S143 Phosphorylation P17612 (PRKACA)
Y144 Phosphorylation
S147 Phosphorylation
S148 Phosphorylation
S151 Phosphorylation
T166 Phosphorylation
S167 Phosphorylation Q16539 (MAPK14) , Q15759 (MAPK11)
S170 Phosphorylation
S190 Phosphorylation
K234 Ubiquitination
S262 Phosphorylation
S265 Phosphorylation
Y286 Phosphorylation P12931 (SRC)
Y288 Phosphorylation P12931 (SRC)
S303 Phosphorylation Q13131 (PRKAA1)
K307 Ubiquitination
K309 Ubiquitination
S313 Phosphorylation Q13131 (PRKAA1)
S318 Phosphorylation
S378 Phosphorylation
T429 Phosphorylation
T432 Phosphorylation
S436 Phosphorylation
S441 Phosphorylation
S443 Phosphorylation
T455 Phosphorylation
K458 Acetylation
S461 Phosphorylation
T467 Phosphorylation
T469 Phosphorylation
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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