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  • 名 称:
    磷酸化 FANCA (Ser1449) 抗体
  • 货 号:
    AF7113
  • RRID:
    AB_2843553
  • 来 源:
    Rabbit
  • 应 用:
    WB,IHC,IF/ICC,ELISA(peptide)
  • 反 应:
    Human, Mouse, Rat
  • 预 测:
    Bovine, Horse
  • 蛋白号:
  • 分子量:
    162kD;
    163kDa(Calculated).
  • 浓 度:
    1mg/ml
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产品信息

别名:Expand▼

FA 1; FA; FA H; FA1; FAA; FACA; FAH; Fanca; FANCA_HUMAN; FANCH; Fanconi anemia complementation group A; Fanconi anemia complementation group H; Fanconi anemia group A protein; Fanconi anemia type 1; MGC75158; Protein FACA;

应用:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.

反应性:

Human, Mouse, Rat

预测反应:

Bovine(80%), Horse(80%)

来源:

Rabbit

克隆性:

Polyclonal

纯化:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

特异性:

Phospho-FANCA (Ser1449) Antibody detects endogenous levels of FANCA only when phosphorylated at Ser1449.

RRID:

AB_2843553
引用格式: Affinity Biosciences Cat# AF7113, RRID:AB_2843553.

形式:

Liquid

浓度:

1mg/ml

保存条件:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

免疫原

Immunogen:

A synthesized peptide derived from human FANCA around the phosphorylation site of Ser1449.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

FANCA

Molecular Weight:

Observed Mol.Wt.: 162kD.
Predicted Mol.Wt.: 163kDa(Calculated)..

Subcellular Location:

Nucleus. Cytoplasm. The major form is nuclear. The minor form is cytoplasmic.

Sequence:
MSDSWVPNSASGQDPGGRRRAWAELLAGRVKREKYNPERAQKLKESAVRLLRSHQDLNALLLEVEGPLCKKLSLSKVIDCDSSEAYANHSSSFIGSALQDQASRLGVPVGILSAGMVASSVGQICTAPAETSHPVLLTVEQRKKLSSLLEFAQYLLAHSMFSRLSFCQELWKIQSSLLLEAVWHLHVQGIVSLQELLESHPDMHAVGSWLFRNLCCLCEQMEASCQHADVARAMLSDFVQMFVLRGFQKNSDLRRTVEPEKMPQVTVDVLQRMLIFALDALAAGVQEESSTHKIVRCWFGVFSGHTLGSVISTDPLKRFFSHTLTQILTHSPVLKASDAVQMQREWSFARTHPLLTSLYRRLFVMLSAEELVGHLQEVLETQEVHWQRVLSFVSALVVCFPEAQQLLEDWVARLMAQAFESCQLDSMVTAFLVVRQAALEGPSAFLSYADWFKASFGSTRGYHGCSKKALVFLFTFLSELVPFESPRYLQVHILHPPLVPGKYRSLLTDYISLAKTRLADLKVSIENMGLYEDLSSAGDITEPHSQALQDVEKAIMVFEHTGNIPVTVMEASIFRRPYYVSHFLPALLTPRVLPKVPDSRVAFIESLKRADKIPPSLYSTYCQACSAAEEKPEDAALGVRAEPNSAEEPLGQLTAALGELRASMTDPSQRDVISAQVAVISERLRAVLGHNEDDSSVEISKIQLSINTPRLEPREHMAVDLLLTSFCQNLMAASSVAPPERQGPWAALFVRTMCGRVLPAVLTRLCQLLRHQGPSLSAPHVLGLAALAVHLGESRSALPEVDVGPPAPGAGLPVPALFDSLLTCRTRDSLFFCLKFCTAAISYSLCKFSSQSRDTLCSCLSPGLIKKFQFLMFRLFSEARQPLSEEDVASLSWRPLHLPSADWQRAALSLWTHRTFREVLKEEDVHLTYQDWLHLELEIQPEADALSDTERQDFHQWAIHEHFLPESSASGGCDGDLQAACTILVNALMDFHQSSRSYDHSENSDLVFGGRTGNEDIISRLQEMVADLELQQDLIVPLGHTPSQEHFLFEIFRRRLQALTSGWSVAASLQRQRELLMYKRILLRLPSSVLCGSSFQAEQPITARCEQFFHLVNSEMRNFCSHGGALTQDITAHFFRGLLNACLRSRDPSLMVDFILAKCQTKCPLILTSALVWWPSLEPVLLCRWRRHCQSPLPRELQKLQEGRQFASDFLSPEAASPAPNPDWLSAAALHFAIQQVREENIRKQLKKLDCEREELLVFLFFFSLMGLLSSHLTSNSTTDLPKAFHVCAAILECLEKRKISWLALFQLTESDLRLGRLLLRVAPDQHTRLLPFAFYSLLSYFHEDAAIREEAFLHVAVDMYLKLVQLFVAGDTSTVSPPAGRSLELKGQGNPVELITKARLFLLQLIPRCPKKSFSHVAELLADRGDCDPEVSAALQSRQQAAPDADLSQEPHLF

研究背景

Function:

DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be involved in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability.

Post-translational Modifications:

Phosphorylation is required for the formation of the nuclear complex. Not phosphorylated in cells derived from groups A, B, C, E, F, G, and H.

Subcellular Location:

Nucleus. Cytoplasm.
Note: The major form is nuclear. The minor form is cytoplasmic.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location

Subunit Structure:

Belongs to the multisubunit FA complex composed of FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9 and FANCM. The complex is not found in FA patients. In complex with FANCF, FANCG and FANCL, but not with FANCC, nor FANCE, interacts with HES1; this interaction may be essential for the stability and nuclear localization of FA core complex proteins. The complex with FANCC and FANCG may also include EIF2AK2 and HSP70. Interacts with FAAP20/C1orf86; interaction is direct.

研究领域

Research Fields:

· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.

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Catalog Number :

AF7113-BP
(Blocking peptide available as AF7113-BP)

Price/Size :

¥2000/1mg.
Tips: For modified antibodies, we provide modified peptides(0.5mg) and non-modified peptides(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Horse
80%
Bovine
80%
Pig
70%
Sheep
70%
Dog
0%
Xenopus
0%
Zebrafish
0%
Chicken
0%
Rabbit
0%
High similarity Medium similarity Low similarity No similarity
O15360 as Substrate
Site PTM Type Enzyme
K34 Ubiquitination
K44 Ubiquitination
K70 Ubiquitination
K76 Ubiquitination
S165 Phosphorylation
K249 Ubiquitination
T256 Phosphorylation
K261 Ubiquitination
K293 Ubiquitination
S303 Phosphorylation
S309 Phosphorylation
T323 Phosphorylation
T329 Phosphorylation
K335 Ubiquitination
S337 Phosphorylation
S347 Phosphorylation Q13131 (PRKAA1)
T351 Phosphorylation P51955 (NEK2)
S357 Phosphorylation
S421 Phosphorylation
S458 Phosphorylation
Y488 Phosphorylation
K515 Ubiquitination
K522 Ubiquitination
K608 Ubiquitination
K612 Ubiquitination
K631 Ubiquitination
R683 Methylation
K701 Ubiquitination
S849 Phosphorylation
S850 Phosphorylation
S858 Phosphorylation
K921 Sumoylation
K1079 Ubiquitination
S1149 Phosphorylation P31749 (AKT1)
K1199 Ubiquitination
S1208 Phosphorylation
S1301 Phosphorylation
T1309 Phosphorylation
K1387 Ubiquitination
K1398 Ubiquitination
K1413 Ubiquitination
S1449 Phosphorylation Q13315 (ATM) , Q13535 (ATR)
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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