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  • 名 称:
    MRCKbeta 抗体
  • 货 号:
    AF9116
  • RRID:
    AB_2843306
  • 来 源:
    Rabbit
  • 应 用:
    WB,IHC,IF/ICC,ELISA(peptide)
  • 反 应:
    Human
  • 预 测:
    Pig(100%), Zebrafish(82%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)
  • 蛋白号:
  • 分子量:
    194kD;
    194kDa(Calculated).
  • 浓 度:
    1mg/ml
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产品信息

别名:Expand▼

CDC42 binding protein kinase beta; CDC42-binding protein kinase beta; CDC42BPB; DMPK like beta; DMPK-like beta; KIAA1124; MRCK beta; MRCKB_HUMAN; Myotonic dystrophy kinase related CDC42 binding kinase beta; Myotonic dystrophy kinase-related CDC42-binding kinase beta; Myotonic dystrophy protein kinase like beta; Myotonic dystrophy protein kinase-like beta; Serine/threonine protein kinase MRCK beta; Serine/threonine-protein kinase MRCK beta;

应用:

WB 1:1000, IHC 1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.

反应性:

Human

预测反应:

Pig(100%), Zebrafish(82%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)

来源:

Rabbit

克隆性:

Polyclonal

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

特异性:

MRCKbeta Antibody detects endogenous levels of total MRCKbeta.

免疫原:

A synthesized peptide derived from human MRCKbeta, corresponding to a region within C-terminal amino acids.

RRID:

AB_2843306
引用格式: Affinity Biosciences Cat# AF9116, RRID:AB_2843306.

形式:

Liquid

浓度:

1mg/ml

保存条件:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

免疫原

Immunogen:

A synthesized peptide derived from human MRCKbeta, corresponding to a region within C-terminal amino acids.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

CDC42BPB

Molecular Weight:

Observed Mol.Wt.: 194kD.
Predicted Mol.Wt.: 194kDa(Calculated)..

Subcellular Location:

Cytoplasm. Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell-cell junction. This concentration is PH-domain dependent.

Tissue Specificity:

Q9Y5S2 MRCKB_HUMAN:
Expressed in all tissues examined, with high levels in heart, brain, placenta and lung.

Sequence:
MSAKVRLKKLEQLLLDGPWRNESALSVETLLDVLVCLYTECSHSALRRDKYVAEFLEWAKPFTQLVKEMQLHREDFEIIKVIGRGAFGEVAVVKMKNTERIYAMKILNKWEMLKRAETACFREERDVLVNGDCQWITALHYAFQDENHLYLVMDYYVGGDLLTLLSKFEDKLPEDMARFYIGEMVLAIDSIHQLHYVHRDIKPDNVLLDVNGHIRLADFGSCLKMNDDGTVQSSVAVGTPDYISPEILQAMEDGMGKYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHEERFQFPSHVTDVSEEAKDLIQRLICSRERRLGQNGIEDFKKHAFFEGLNWENIRNLEAPYIPDVSSPSDTSNFDVDDDVLRNTEILPPGSHTGFSGLHLPFIGFTFTTESCFSDRGSLKSIMQSNTLTKDEDVQRDLEHSLQMEAYERRIRRLEQEKLELSRKLQESTQTVQSLHGSSRALSNSNRDKEIKKLNEEIERLKNKIADSNRLERQLEDTVALRQEREDSTQRLRGLEKQHRVVRQEKEELHKQLVEASERLKSQAKELKDAHQQRKLALQEFSELNERMAELRAQKQKVSRQLRDKEEEMEVATQKVDAMRQEMRRAEKLRKELEAQLDDAVAEASKERKLREHSENFCKQMESELEALKVKQGGRGAGATLEHQQEISKIKSELEKKVLFYEEELVRREASHVLEVKNVKKEVHDSESHQLALQKEILMLKDKLEKSKRERHNEMEEAVGTIKDKYERERAMLFDENKKLTAENEKLCSFVDKLTAQNRQLEDELQDLAAKKESVAHWEAQIAEIIQWVSDEKDARGYLQALASKMTEELEALRSSSLGSRTLDPLWKVRRSQKLDMSARLELQSALEAEIRAKQLVQEELRKVKDANLTLESKLKDSEAKNRELLEEMEILKKKMEEKFRADTGLKLPDFQDSIFEYFNTAPLAHDLTFRTSSASEQETQAPKPEASPSMSVAASEQQEDMARPPQRPSAVPLPTTQALALAGPKPKAHQFSIKSFSSPTQCSHCTSLMVGLIRQGYACEVCSFACHVSCKDGAPQVCPIPPEQSKRPLGVDVQRGIGTAYKGHVKVPKPTGVKKGWQRAYAVVCDCKLFLYDLPEGKSTQPGVIASQVLDLRDDEFSVSSVLASDVIHATRRDIPCIFRVTASLLGAPSKTSSLLILTENENEKRKWVGILEGLQSILHKNRLRNQVVHVPLEAYDSSLPLIKAILTAAIVDADRIAVGLEEGLYVIEVTRDVIVRAADCKKVHQIELAPREKIVILLCGRNHHVHLYPWSSLDGAEGSFDIKLPETKGCQLMATATLKRNSGTCLFVAVKRLILCYEIQRTKPFHRKFNEIVAPGSVQCLAVLRDRLCVGYPSGFCLLSIQGDGQPLNLVNPNDPSLAFLSQQSFDALCAVELESEEYLLCFSHMGLYVDPQGRRARAQELMWPAAPVACSCSPTHVTVYSEYGVDVFDVRTMEWVQTIGLRRIRPLNSEGTLNLLNCEPPRLIYFKSKFSGAVLNVPDTSDNSKKQMLRTRSKRRFVFKVPEEERLQQRREMLRDPELRSKMISNPTNFNHVAHMGPGDGMQVLMDLPLSAVPPSQEERPGPAPTNLARQPPSRNKPYISWPSSGGSEPSVTVPLRSMSDPDQDFDKEPDSDSTKHSTPSNSSNPSGPPSPNSPHRSQLPLEGLEQPACDT

研究背景

Function:

Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2. In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration. Phosphorylates PPP1R12A. In concert with FAM89B/LRAP25 mediates the targeting of LIMK1 to the lamellipodium resulting in its activation and subsequent phosphorylation of CFL1 which is important for lamellipodial F-actin regulation (By similarity).

Post-translational Modifications:

Proteolytically cleaved by caspases upon apoptosis induction.

Subcellular Location:

Cytoplasm. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cell junction. Cell projection>Lamellipodium.
Note: Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell-cell junction. This concentration is PH-domain dependent (By similarity). Detected at the leading edge of migrating cells. Localization at the leading edge of migrating cells requires interaction with catalytically active CDC42 (PubMed:21240187). Localizes in the lamellipodium in a FAM89B/LRAP25-dependent manner (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location

Tissue Specificity:

Expressed in all tissues examined, with high levels in heart, brain, placenta and lung.

Subunit Structure:

Homodimer and homotetramer via the coiled coil regions. Interacts tightly with GTP-bound but not GDP-bound CDC42. Interacts with TJP1, when in the presence of catalytically active CDC42 (By similarity). Forms a tripartite complex with MYO18A and LURAP1 with the latter acting as an adapter connecting CDC42BPB and MYO18A. LURAP1 binding results in activation of CDC42BPB by abolition of its negative autoregulation. Interacts with STRIP1, STRN3 and SIKE1. Interacts with CPNE4 (via VWFA domain). Interacts with LURAP1. Interacts (via AGC-kinase C-terminal domain) with FAM89B/LRAP25 (via LRR repeat). Forms a tripartite complex with FAM89B/LRAP25 and LIMK1 (By similarity).

Similarity:

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily.

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Catalog Number :

AF9116-BP
(Blocking peptide available as AF9116-BP)

Price/Size :

¥2000/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Pig
100%
Sheep
100%
Dog
100%
Bovine
100%
Horse
100%
Zebrafish
82%
Rabbit
0%
Xenopus
0%
Chicken
0%
High similarity Medium similarity Low similarity No similarity
Q9Y5S2 as Substrate
Site PTM Type Enzyme
S304 Phosphorylation
T307 Phosphorylation
S310 Phosphorylation
K314 Ubiquitination
K337 Ubiquitination
S363 Phosphorylation
S417 Phosphorylation
S421 Phosphorylation
T423 Phosphorylation
K426 Ubiquitination
K454 Ubiquitination
K460 Ubiquitination
T467 Phosphorylation
S474 Phosphorylation
S481 Phosphorylation
K489 Acetylation
S524 Phosphorylation
K571 Ubiquitination
S641 Phosphorylation
K642 Ubiquitination
S659 Phosphorylation
K665 Ubiquitination
R671 Methylation
T676 Phosphorylation
S707 Phosphorylation
T757 Phosphorylation
K789 Ubiquitination
K807 Ubiquitination
S840 Phosphorylation
T843 Phosphorylation
S851 Phosphorylation
S852 Phosphorylation
T858 Phosphorylation
K864 Ubiquitination
S868 Phosphorylation
K870 Ubiquitination
K890 Ubiquitination
K910 Ubiquitination
S914 Phosphorylation
K929 Ubiquitination
Y954 Phosphorylation
S1029 Phosphorylation
K1083 Ubiquitination
K1099 Ubiquitination
Y1118 Phosphorylation
K1135 Ubiquitination
T1196 Phosphorylation
K1202 Ubiquitination
K1280 Ubiquitination
K1337 Methylation
K1337 Ubiquitination
Y1355 Phosphorylation
K1526 Methylation
K1528 Methylation
K1528 Ubiquitination
S1530 Phosphorylation
K1544 Ubiquitination
K1559 Ubiquitination
Y1638 Phosphorylation
S1640 Phosphorylation
S1643 Phosphorylation
S1644 Phosphorylation
S1647 Phosphorylation
S1650 Phosphorylation
T1652 Phosphorylation
S1659 Phosphorylation
S1677 Phosphorylation
T1678 Phosphorylation
S1680 Phosphorylation
S1682 Phosphorylation
S1686 Phosphorylation
S1690 Phosphorylation
S1693 Phosphorylation
C1709 S-Nitrosylation
Q9Y5S2 as PTM Enzyme
Substrate Site Source
O14974 (PPP1R12A) S852 Uniprot
P24844 (MYL9) S20 Uniprot
P26038 (MSN) T558 Uniprot
P35611 (ADD1) T445 Uniprot
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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