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  • 名 称:
    MCP-1 抗体
  • 货 号:
    DF7577
  • 来 源:
    Rabbit
  • 应 用:
    WB,IHC,IF/ICC,ELISA
  • 反 应:
    Human, Mouse, Rat
  • 预 测:
    Horse(83%)
  • 蛋白号:
  • 分子量:
    14 kDa
  • 浓 度:
    1mg/ml
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产品信息

Alternative Names:Expand▼

C-C motif chemokine 2; CCL2; CCL2_HUMAN; Chemokine (C C motif) ligand 2; GDCF 2; GDCF-2; GDCF2; HC11; HSMCR30; JE; MCAF; MCP 1; MCP-1; MCP1; MGC9434; Monocyte chemoattractant protein 1; Monocyte chemotactic and activating factor; Monocyte chemotactic protein 1; Monocyte secretory protein JE; SCYA2; Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je); Small inducible cytokine A2; Small inducible cytokine subfamily A (Cys Cys), member 2; Small-inducible cytokine A2; SMC CF; SMC-CF; SMCCF;

Applications:

WB 1:1000-3000, IHC 1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Reactivity:

Human, Mouse, Rat

Predicted Reactivity:

Horse(83%)

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity:

MCP-1 Antibody detects endogenous levels of total MCP-1.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

免疫原

Immunogen:

A synthesized peptide derived from human MCP-1, corresponding to a region within the internal amino acids.

Uniprot:



>>Visit The Human Protein Atlas

Gene id:

Molecular Weight:

Observed Mol.Wt.: 14 kDa.
Predicted Mol.Wt.: 12kDa.

Subcellular Location:

Secreted.

Tissue Specificity:

Expressed in the seminal plasma, endometrial fluid and follicular fluid (at protein level).

Sequence:
        10         20         30         40         50
MKVSAALLCL LLIAATFIPQ GLAQPDAINA PVTCCYNFTN RKISVQRLAS
60 70 80 90
YRRITSSKCP KEAVIFKTIV AKEICADPKQ KWVQDSMDHL DKQTQTPKT

研究背景

Function:

Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.

Post-translational Modifications:

Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.

Subcellular Location:

Extracellular region or secreted;

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Subunit Structure:

Monomer or homodimer; in equilibrium. Binds to CCR2 and CCR4. Is tethered on endothelial cells by glycosaminoglycan (GAG) side chains of proteoglycans.

Similarity:

Belongs to the intercrine beta (chemokine CC) family.

研究领域

Research Fields:

· Environmental Information Processing > Signal transduction > TNF signaling pathway.(View pathway)
· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.(View pathway)
· Human Diseases > Immune diseases > Rheumatoid arthritis.
· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.
· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).
· Human Diseases > Infectious diseases: Viral > Influenza A.
· Human Diseases > Infectious diseases: Parasitic > Malaria.
· Organismal Systems > Immune system > IL-17 signaling pathway.(View pathway)
· Organismal Systems > Immune system > NOD-like receptor signaling pathway.(View pathway)
· Organismal Systems > Immune system > Chemokine signaling pathway.(View pathway)

Western blot analysis of extracts from Rat testis, using MCP-1 Antibody. The lane on the left was treated with blocking peptide.
Western blot analysis of MCP-1 expression in Mouse brain lysates
Western blot analysis of MCP-1 using HUVEC whole cell lysates
DF7577 at 1/100 staining Human melanoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Urinary MCP‑1, HMGB1 increased in calcium nephrolithiasis patients and the influence of hypercalciuria on the production of the two cytokines Yang Wang1 · Chun Sun1 · Chengyang Li1 · Yaoliang Deng1 · Guohua Zeng2 · Zhiwei Tao1 · Xiang Wang1 · Xiaofeng Guan1 · Yutong Zhao
DF7577 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(DF7577 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.
IF/ICC analysis mouse intestinal tissue using MCP-1 Antibody(Green). Blue: DAPI.

Reference Citations:

1). Yang Z et al. CCL2/CCR2 Axis Promotes the Progression of Salivary Adenoid Cystic Carcinoma via Recruiting and Reprogramming the Tumor-Associated Macrophages. Front Oncol 2019 Apr 9;9:231 (PubMed: 31024838) [IF=4.137]

2). Li Z et al. Melatonin therapy protects against renal injury before and after release of bilateral ureteral obstruction in rats. Life Sci 2019 May 14 (PubMed: 31100324) [IF=3.448]

3). Ye JS et al. SIRT3 activator honokiol ameliorates surgery/anesthesia-induced cognitive decline in mice through anti-oxidative stress and anti-inflammatory in hippocampus. CNS Neurosci Ther 2019 Mar;25(3):355-366 (PubMed: 30296006) [IF=3.394]

4). Ye JS et al. SIRT3 activator honokiol ameliorates surgery/anesthesia-induced cognitive decline in mice through anti-oxidative stress and anti-inflammatory in hippocampus. CNS Neurosci Ther 2019 Mar;25(3):355-366 (PubMed: 30296006) [IF=3.394]

5). Ye JS et al. SIRT3 activator honokiol ameliorates surgery/anesthesia-induced cognitive decline in mice through anti-oxidative stress and anti-inflammatory in hippocampus. CNS Neurosci Ther 2019 Mar;25(3):355-366 (PubMed: 30296006) [IF=3.394]

6). Chen Z et al. Pioglitazone decreased renal calcium oxalate crystal formation by suppressing M1-macrophage polarization via PPARγ-microRNA-23 axis. Am J Physiol Renal Physiol 2019 May 15 (PubMed: 31091119) [IF=3.176]

7). Qin X et al. An Extracellular Matrix-Mimicking Hydrogel for Full Thickness Wound Healing in Diabetic Mice. Macromol Biosci 2018 Jul;18(7):e1800047 (PubMed: 29737012)

8). Wang Y et al. Urinary MCP-1、HMGB1 increased in calcium nephrolithiasis patients and the influence of hypercalciuria on the production of the two cytokines. Urolithiasis 2017 Apr;45(2):159-175 (PubMed: 27393275)

Application: IHC    Species:human;    Sample:Not available

Fig. 1 Immunostaining localization for MCP-1 in kidney (magnification ×400). a, c, e Group E-IHC; b, d, f group C-IHC. Glo glomerulus, PCT proximal-convoluted tubule, DCT distal-convoluted tubule, CD collecting duct epithelial cells, black arrows thin segment of Henle’s loop


9). Xia Y et al. Expression and significance of Sirt1 in renal allografts at the early stage of chronic renal allograft dysfunction. Transpl Immunol 2018 Jun;48:18-25 (PubMed: 29452170)

10). Pavlyashik GV et al. [Comparative estimation of antilithogenic activity of porcine kidney derived biomedical substance and sodium citrate in experimental urolithiasis]. Urologiia 2017 Jun;(2):24-27 (PubMed: 28631902)

11). et al. Inhibition of miR-155-5p Exerts Anti-Fibrotic Effects in Silicotic Rats by Regulating Meprin α.

12). Ding M et al. MCP-1/CCL2 Mediated by Autocrine Loop of PDGF-BB Promotes Invasion of Lung Cancer Cell by Recruitment of Macrophages Via CCL2-CCR2 Axis. J Interferon Cytokine Res 2019 Feb 7 (PubMed: 30730243)

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Catalog Number :

DF7577-BP
(Blocking peptide available as DF7577-BP)

Price/Size :

¥2000/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Horse
83%
Zebrafish
0%
Chicken
0%
Rabbit
0%
Xenopus
0%
Dog
0%
Bovine
0%
Sheep
0%
Pig
0%
High similarity Medium similarity Low similarity No similarity
P13500 as Substrate
Site PTM Type Enzyme
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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