Affinity-Purified phospho-specific polyclonal antibody production $1199/ project(From Peptide Synthesis to ELISA titer assay)
Polyclonal phospho-specific Antibody Development Schedules
Phase |
Time |
I. Peptide synthesis |
2 week |
II. Conjugation |
|
III. Immunization and anti-sera production
|
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Ⅳ.Peptide affinity purification and ELISA test. Separating the phosphorylated antibodies from the non-phosphorylated antibodies is performed by immuno-affinity purification, using two columns ,which does not cross-react with non-phosphospecific peptides (confirmed by ELISA) |
2 weeks |
|
|
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